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Whole transcriptome RNA sequencing on bone marrow and peripheral blood samples from patients with acute myeloid leukemia at diagnosis or relapse.

One hundred cryopreserved bone marrow and peripheral blood samples from patients with acute myeloid leukemia (AML) with 10-90% blasts were selected from the biobank of the Department of Hematology of Leiden University Medical Center (LUMC). The AML cases cover all subtypes, and specifically include known subtype-defining balanced chromosomal translocations according to the WHO classification. The samples were obtained from 96 patients and include three pairs of de novo and relapsed AML and one pair of de novo and presumed therapy-related AML (tAML). Total RNA was isolated from mononuclear cells without prior enrichment for leukemic blasts. The quality and integrity of total RNA was checked and RNA libraries were prepared using the TruSeq RNA library preparation kit v2 (Illumina, San Diego, CA) in an ISO/IEC 17025-accredited protocol. This workflow started with enrichment of messenger RNA by oligo dT magnetic beads. After fragmentation, cDNA synthesis was performed, followed by adaptor ligation and PCR amplification. Paired-end sequencing with a read length of 126 bp was performed on an Illumina HiSeq 2500 v4 sequencer to at least 12.5 Gbp per sample. Image analysis, base calling, and quality check was performed with Illumina data analysis pipeline RTA v1.18.64 and Bcl2fastq v1.8.4. RNAseq reads are provided in compressed Sanger FASTQ format.

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Policy: Whole transcriptome RNA sequencing as comprehensive diagnostic tool for acute myeloid leukemia.

Restricted access because the study concerns patient data which should not be traced back to the patients. We will require citation plus acknowledgement.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001003096 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00002044247 fastq.gz 4.6 GB
EGAF00002044248 fastq.gz 4.7 GB
EGAF00002044249 fastq.gz 4.5 GB
EGAF00002044250 fastq.gz 4.6 GB
EGAF00002044251 fastq.gz 4.5 GB
EGAF00002044252 fastq.gz 4.6 GB
EGAF00002044253 fastq.gz 4.9 GB
EGAF00002044254 fastq.gz 5.1 GB
EGAF00002044255 fastq.gz 5.1 GB
EGAF00002044256 fastq.gz 5.3 GB
EGAF00002044257 fastq.gz 5.7 GB
EGAF00002044258 fastq.gz 6.1 GB
EGAF00002044259 fastq.gz 4.7 GB
EGAF00002044260 fastq.gz 4.7 GB
EGAF00002044261 fastq.gz 5.6 GB
EGAF00002044262 fastq.gz 5.6 GB
EGAF00002044263 fastq.gz 5.0 GB
EGAF00002044264 fastq.gz 4.8 GB
EGAF00002044265 fastq.gz 4.8 GB
EGAF00002044266 fastq.gz 4.9 GB
EGAF00002044267 fastq.gz 5.1 GB
EGAF00002044268 fastq.gz 5.0 GB
EGAF00002044269 fastq.gz 4.6 GB
EGAF00002044270 fastq.gz 4.5 GB
EGAF00002044271 fastq.gz 5.3 GB
EGAF00002044272 fastq.gz 5.3 GB
EGAF00002044273 fastq.gz 5.5 GB
EGAF00002044274 fastq.gz 5.4 GB
EGAF00002044275 fastq.gz 4.5 GB
EGAF00002044276 fastq.gz 4.5 GB
EGAF00002044277 fastq.gz 5.0 GB
EGAF00002044278 fastq.gz 5.0 GB
EGAF00002044279 fastq.gz 4.3 GB
EGAF00002044280 fastq.gz 4.3 GB
EGAF00002044281 fastq.gz 5.6 GB
EGAF00002044282 fastq.gz 5.6 GB
EGAF00002044283 fastq.gz 6.1 GB
EGAF00002044284 fastq.gz 6.2 GB
EGAF00002044285 fastq.gz 5.8 GB
EGAF00002044286 fastq.gz 5.6 GB
EGAF00002044287 fastq.gz 5.3 GB
EGAF00002044288 fastq.gz 5.3 GB
EGAF00002044289 fastq.gz 4.4 GB
EGAF00002044290 fastq.gz 4.4 GB
EGAF00002044291 fastq.gz 5.4 GB
EGAF00002044292 fastq.gz 5.3 GB
EGAF00002044293 fastq.gz 4.5 GB
EGAF00002044294 fastq.gz 4.4 GB
EGAF00002044295 fastq.gz 5.2 GB
EGAF00002044296 fastq.gz 5.1 GB
EGAF00002044297 fastq.gz 7.1 GB
EGAF00002044298 fastq.gz 7.1 GB
EGAF00002044299 fastq.gz 5.7 GB
EGAF00002044300 fastq.gz 5.8 GB
EGAF00002044301 fastq.gz 6.2 GB
EGAF00002044302 fastq.gz 6.1 GB
EGAF00002044303 fastq.gz 4.4 GB
EGAF00002044304 fastq.gz 4.5 GB
EGAF00002044305 fastq.gz 6.1 GB
EGAF00002044306 fastq.gz 6.3 GB
EGAF00002044307 fastq.gz 5.5 GB
EGAF00002044308 fastq.gz 5.6 GB
EGAF00002044309 fastq.gz 6.0 GB
EGAF00002044310 fastq.gz 6.1 GB
EGAF00002044311 fastq.gz 5.9 GB
EGAF00002044312 fastq.gz 6.0 GB
EGAF00002044313 fastq.gz 4.3 GB
EGAF00002044314 fastq.gz 4.4 GB
EGAF00002044315 fastq.gz 6.0 GB
EGAF00002044316 fastq.gz 6.1 GB
EGAF00002044317 fastq.gz 4.4 GB
EGAF00002044318 fastq.gz 4.5 GB
EGAF00002044319 fastq.gz 4.8 GB
EGAF00002044320 fastq.gz 4.8 GB
EGAF00002044321 fastq.gz 4.3 GB
EGAF00002044322 fastq.gz 4.3 GB
EGAF00002044323 fastq.gz 5.0 GB
EGAF00002044324 fastq.gz 4.9 GB
EGAF00002044325 fastq.gz 7.3 GB
EGAF00002044326 fastq.gz 7.3 GB
EGAF00002044327 fastq.gz 5.5 GB
EGAF00002044328 fastq.gz 5.7 GB
EGAF00002044329 fastq.gz 6.0 GB
EGAF00002044330 fastq.gz 6.1 GB
EGAF00002044331 fastq.gz 4.8 GB
EGAF00002044332 fastq.gz 4.7 GB
EGAF00002044333 fastq.gz 4.1 GB
EGAF00002044334 fastq.gz 4.1 GB
EGAF00002044335 fastq.gz 4.6 GB
EGAF00002044336 fastq.gz 4.6 GB
EGAF00002044337 fastq.gz 6.4 GB
EGAF00002044338 fastq.gz 6.2 GB
EGAF00002044339 fastq.gz 4.3 GB
EGAF00002044340 fastq.gz 4.4 GB
EGAF00002044341 fastq.gz 4.2 GB
EGAF00002044342 fastq.gz 4.1 GB
EGAF00002044343 fastq.gz 4.7 GB
EGAF00002044344 fastq.gz 4.7 GB
EGAF00002044345 fastq.gz 4.2 GB
EGAF00002044346 fastq.gz 4.3 GB
EGAF00002044347 fastq.gz 4.9 GB
EGAF00002044348 fastq.gz 4.8 GB
EGAF00002044349 fastq.gz 4.2 GB
EGAF00002044350 fastq.gz 4.1 GB
EGAF00002044351 fastq.gz 4.3 GB
EGAF00002044352 fastq.gz 4.3 GB
EGAF00002044353 fastq.gz 4.2 GB
EGAF00002044354 fastq.gz 4.2 GB
EGAF00002044355 fastq.gz 4.1 GB
EGAF00002044356 fastq.gz 4.2 GB
EGAF00002044357 fastq.gz 4.5 GB
EGAF00002044358 fastq.gz 4.6 GB
EGAF00002044359 fastq.gz 4.9 GB
EGAF00002044360 fastq.gz 5.1 GB
EGAF00002044361 fastq.gz 6.3 GB
EGAF00002044362 fastq.gz 6.2 GB
EGAF00002044363 fastq.gz 4.8 GB
EGAF00002044364 fastq.gz 4.7 GB
EGAF00002044365 fastq.gz 4.7 GB
EGAF00002044366 fastq.gz 4.6 GB
EGAF00002044367 fastq.gz 4.5 GB
EGAF00002044368 fastq.gz 4.6 GB
EGAF00002044369 fastq.gz 4.5 GB
EGAF00002044370 fastq.gz 4.4 GB
EGAF00002044371 fastq.gz 4.6 GB
EGAF00002044372 fastq.gz 4.4 GB
EGAF00002044373 fastq.gz 4.7 GB
EGAF00002044374 fastq.gz 4.8 GB
EGAF00002044375 fastq.gz 4.9 GB
EGAF00002044376 fastq.gz 4.9 GB
EGAF00002044377 fastq.gz 4.7 GB
EGAF00002044378 fastq.gz 4.7 GB
EGAF00002044379 fastq.gz 6.6 GB
EGAF00002044380 fastq.gz 6.5 GB
EGAF00002044381 fastq.gz 6.0 GB
EGAF00002044382 fastq.gz 6.2 GB
EGAF00002044383 fastq.gz 4.5 GB
EGAF00002044384 fastq.gz 4.5 GB
EGAF00002044385 fastq.gz 4.6 GB
EGAF00002044386 fastq.gz 4.6 GB
EGAF00002044387 fastq.gz 5.3 GB
EGAF00002044388 fastq.gz 5.4 GB
EGAF00002044389 fastq.gz 5.1 GB
EGAF00002044390 fastq.gz 5.0 GB
EGAF00002044391 fastq.gz 4.7 GB
EGAF00002044392 fastq.gz 4.7 GB
EGAF00002044393 fastq.gz 4.6 GB
EGAF00002044394 fastq.gz 4.7 GB
EGAF00002044395 fastq.gz 5.6 GB
EGAF00002044396 fastq.gz 5.5 GB
EGAF00002044397 fastq.gz 5.4 GB
EGAF00002044398 fastq.gz 5.4 GB
EGAF00002044399 fastq.gz 4.6 GB
EGAF00002044400 fastq.gz 4.5 GB
EGAF00002044401 fastq.gz 5.0 GB
EGAF00002044402 fastq.gz 5.0 GB
EGAF00002044403 fastq.gz 4.7 GB
EGAF00002044404 fastq.gz 4.8 GB
EGAF00002044405 fastq.gz 4.8 GB
EGAF00002044406 fastq.gz 4.9 GB
EGAF00002044407 fastq.gz 4.4 GB
EGAF00002044408 fastq.gz 4.7 GB
EGAF00002044409 fastq.gz 4.6 GB
EGAF00002044410 fastq.gz 4.6 GB
EGAF00002044411 fastq.gz 5.0 GB
EGAF00002044412 fastq.gz 4.9 GB
EGAF00002044413 fastq.gz 5.1 GB
EGAF00002044414 fastq.gz 5.0 GB
EGAF00002044415 fastq.gz 5.8 GB
EGAF00002044416 fastq.gz 6.0 GB
EGAF00002044417 fastq.gz 5.6 GB
EGAF00002044418 fastq.gz 5.7 GB
EGAF00002044419 fastq.gz 5.9 GB
EGAF00002044420 fastq.gz 5.9 GB
EGAF00002044421 fastq.gz 5.5 GB
EGAF00002044422 fastq.gz 5.6 GB
EGAF00002044423 fastq.gz 5.3 GB
EGAF00002044424 fastq.gz 5.3 GB
EGAF00002044425 fastq.gz 5.6 GB
EGAF00002044426 fastq.gz 5.7 GB
EGAF00002044427 fastq.gz 7.7 GB
EGAF00002044428 fastq.gz 7.8 GB
EGAF00002044429 fastq.gz 5.0 GB
EGAF00002044430 fastq.gz 5.1 GB
EGAF00002044431 fastq.gz 6.0 GB
EGAF00002044432 fastq.gz 6.1 GB
EGAF00002044433 fastq.gz 6.0 GB
EGAF00002044434 fastq.gz 6.2 GB
EGAF00002044435 fastq.gz 6.5 GB
EGAF00002044436 fastq.gz 6.5 GB
EGAF00002044437 fastq.gz 6.5 GB
EGAF00002044438 fastq.gz 6.7 GB
EGAF00002044439 fastq.gz 5.3 GB
EGAF00002044440 fastq.gz 5.5 GB
EGAF00002044441 fastq.gz 4.9 GB
EGAF00002044442 fastq.gz 5.0 GB
EGAF00002044443 fastq.gz 5.6 GB
EGAF00002044444 fastq.gz 5.7 GB
EGAF00002044445 fastq.gz 5.8 GB
EGAF00002044446 fastq.gz 6.0 GB
200 Files (1.0 TB)